From: owner-ammf-digest@smoe.org (alt.music.moxy-fruvous digest) To: ammf-digest@smoe.org Subject: alt.music.moxy-fruvous digest V14 #11023 Reply-To: ammf@fruvous.com Sender: owner-ammf-digest@smoe.org Errors-To: owner-ammf-digest@smoe.org Precedence: bulk alt.music.moxy-fruvous digest Tuesday, March 28 2023 Volume 14 : Number 11023 Today's Subjects: ----------------- Pop Your Ear Like THIS And Remove Tinnitus ["Stop Tinnitus" Subject: Pop Your Ear Like THIS And Remove Tinnitus Pop Your Ear Like THIS And Remove Tinnitus http://fedzexsurvey.today/BUc32te3Fp6uigATf4lQ3-HZRH9lUMjZLlPIh8ij11lRLFzXbA http://fedzexsurvey.today/JFNYI-QAfODKN7UovpFoIrfvfenP3lb0-Nlt2gkR-ozcrRAeiw Irreversible inhibitors first form a reversible non-covalent complex with the enzyme (EI or ESI). Subsequently a chemical reaction occurs between the enzyme and inhibitor to produce the covalently modified "dead-end complex" EI* (an irreversible covalent complex). The rate at which EI* is formed is called the inactivation rate or kinact. Since formation of EI may compete with ES, binding of irreversible inhibitors can be prevented by competition either with substrate or with a second, reversible inhibitor. This protection effect is good evidence of a specific reaction of the irreversible inhibitor with the active site. The binding and inactivation steps of this reaction are investigated by incubating the enzyme with inhibitor and assaying the amount of activity remaining over time. The activity will be decreased in a time-dependent manner, usually following exponential decay. Fitting these data to a rate equation gives the rate of inactivation at this concentration of inhibitor. This is done at several different concentrations of inhibitor. If a reversible EI complex is involved the inactivation rate will be saturable and fitting this curve will give kinact and Ki. Another method that is widely used in these analyses is mass spectrometry. Here, accurate measurement of the mass of the unmodified native enzyme and the inactivated enzyme gives the increase in mass caused by reaction with the inhibitor and shows the stoic ------------------------------ End of alt.music.moxy-fruvous digest V14 #11023 ***********************************************